Although many wild and wacky characters exist within the world of Bo-bobo, the story focuses primarily on the nine main heroes who gather together under the most unlikely circumstances to fight against Tsuru Tsurulina the IV and the evil forces of the Maruhage Empire.
At the center of this team is Bobobo-bo Bo-bobo himself, master of "Hanage Shinken" (Fist of the Nosehair) and the completely wacky Hajikelist who will do anything and everything for victory. Through his journey to defeat the forces of baldness, he slowly gathers a team of allies and former enemies who become attached to his cause in one way or another:
Beauty : The teen female who usually has little idea regarding why all the strange stuff is happening.
Don Patch : The egotistical former leader of a group of Hajikelists whose ultimate power comes from his own insanity.
Softon: A warrior with the power of the mysterious land of Babylon, and a head that is made out of ice cream.
Heppokomaru: A teen boy with the stinky abilities of "Onara Shinken" (Fist of the Backwind), and with as little knowledge of what is going on as Beauty knows.
Tokoro Tennosuke: A gelatinous being who is as powerful as he is the consistent target of his allies attacks.
Hatenko: A warrior from Bo-bobo's Hair Kingdom with the power of keys, and an obsession with Don Patch.
Dengaku Man: A cute little mascot who is frequently ignored or left behind.
Torpedo Girl: The transformed state of a former enemy with a short temper and an obsession for teaching her "students" who's boss.
While these nine are the main warriors of the series, others occasionally assist in fighting against the evil in this world, including:
King Nosehair: A nosehair that used to live in Bo-bobo and takes part in the nonsense of "Bo-bobo World".
Serviceman: A covered being that makes everyone see things that shouldn't be seen.
Suzu: A nice girl with psychokinetic powers.
Rice: Master of "Kome Shinken" (Fist of Rice) and powerful Hajikelist.
Hanpen: A walking fishcake who fights with his fists or by jumping a lot.
Despair-kun/Sad Sack: A guy with a bag over his head and serious emotional issues.
Kancho-kun: A tiny being with fingers of fury.
Halekulani: One of Bo-bobo's greatest enemies with a power and obsession tied to his greed.
Bebebe-be Be-bebe: One of Bo-bobo's older brothers and the master of "Sunege Shinken" (Fist of Leghair)
Bububu-bu Bu-bubu: Bo-bobo's sister, master of "Wakige Shinken" (Fist of Armpit Hair) and bearer of a strong resemblance to one of Bo-bobo's fused forms. She also has a crush on Don Patch.
Land Mine Dandy: A skillful Hajikelist and teacher, he is the master of "Enban Shinken" (Fist of the Disc), and is Torpedo Girl's father.
Gunkan/Captain Battleship: User of "self-taught fist of the nose hair" and bobobo's childhood friend.
